Part:BBa_K2771042
Cin inducible Synthase
Quorum sensing is a bacterial communication system that can be very useful for bacterial communication in synthetic biology. In this system, AHL (N-Acyl homoserine lactone) signaling molecules generated in a bacteria binds to a receiver in other bacteria to activate downstream gene expression. The AHL molecules are generated by a synthase and received by other bacteria through a reporter.
Among all the QS pathways, the Cin pathway is based on the production of the 3OC14:1 homoserine lactone (HSL) produced by the Cinl protein. If coupled to a gene with a promoter responsive to quorum sensing signals, this construct can create a response dependant on cell density and L-arabinose concentration. This part contains the Cinl transcribed by a promoter inducible by L-arabinose, permitting for controlled expression of the HSL signal.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1459 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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